Premium
Application of Hierarchical Porous Silica with a Stable Large Porosity for β‐Galactosidase Immobilization
Author(s) -
Bernal Claudia,
Sierra Ligia,
Mesa Monica
Publication year - 2011
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201100174
Subject(s) - porosity , chemical engineering , mesoporous material , kluyveromyces lactis , bromide , adsorption , chemistry , immobilized enzyme , catalysis , mesoporous silica , biocatalysis , silanol , chromatography , materials science , inorganic chemistry , organic chemistry , enzyme , biochemistry , ionic liquid , engineering , gene , saccharomyces cerevisiae
Hierarchical porous silica, particles or monoliths, were synthesized by polycondensation of sodium silicate in the presence of cetyltrimethylammonium bromide and ethyl acetate at different concentrations under hydrothermal conditions. They were used as the support for the immobilization of β‐galactosidase from Kluyveromyces lactis by adsorption. The enzyme loading capacity (higher than 50 mg g −1 support) and the retention ability (lixiviation less than 20 % after 72 h of catalysis) of these supports were explained as a function of the hierarchical porosity, mesopore sizes of 10–40 nm, and macropore sizes of 0.07–20 μm and the presence of ionized silanol groups on the surface. The optimum pH value and temperature for the maximum activity of the obtained hybrid biocatalyst were evaluated, indicating that the three‐dimensional structure of the lactase was not significantly affected during the immobilization process. The stability under extreme conditions was improved in comparison with soluble lactase. The porous supports exhibited morphological and porous stability under the immobilization and catalytic processes. These results show that the obtained materials are good candidates for the immobilization of large enzymes, such as β‐galactosidase.