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Oriented Immobilization of Enzymes Made Fit for Applied Biocatalysis: Non‐Covalent Attachment to Anionic Supports using Z basic2 Module
Author(s) -
Wiesbauer Johanna,
Bolivar Juan M.,
Mueller Mario,
Schiller Margaretha,
Nidetzky Bernd
Publication year - 2011
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201100103
Subject(s) - biocatalysis , covalent bond , enzyme , immobilized enzyme , chemistry , combinatorial chemistry , computer science , nanotechnology , organic chemistry , catalysis , materials science , reaction mechanism
Charge is the message: Non‐covalent attachment to anionic supports (red) through a positively charged Z basic2 (blue) module is a powerful approach for reversible oriented immobilization of enzymes (pI 4‐7) on ion‐exchange resins commonly used in the protein and enzyme technology. High protein loading and near‐native catalytic effectiveness of the carrier‐bound enzyme can be achieved.