Premium
Lipase Congeners Designed by Genetic Code Engineering
Author(s) -
Hoesl Michael G.,
AcevedoRocha Carlos G.,
Nehring Sebastian,
Royter Marina,
Wolschner Christina,
Wiltschi Birgit,
Budisa Nediljko,
Antranikian Garabed
Publication year - 2011
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201000253
Subject(s) - genetic code , amino acid , lipase , protein engineering , biocatalysis , tyrosine , phenylalanine , methionine , synthetic biology , chemistry , enzyme , biochemistry , computational biology , biology , reaction mechanism , catalysis
Classical enzyme optimization exploits the chemistry confined to the 20 canonical amino acids encoded by the standard genetic code. ‘Genetic code engineering’ allows the global substitution of particular residues with synthetic analogues, endowing proteins with chemical diversity not found in nature. These proteins are congeners of the parent protein because they originate from the same gene sequence, but contain a fraction of noncanonical amino acids. Global substitutions of methionine, proline, phenylalanine, and tyrosine have been carried out with related analogues in Thermoanaerobacter thermohydrosulfuricus lipase. This study represents the first extensive report of an important biocatalyst substituted with a high number of noncanonical amino acids. The generated lipase congeners displayed special features such as enhanced activation, elevated enzyme activity (by up to 25 %) and substrate tolerance (by up to 40 %), and changes in optimal temperature (by up to 20 °C) and pH (by up to 3). These emergent features achieved by genetic code engineering might be important not only for academic research, but also for numerous economical applications in the food, detergent, chemical, pharmaceutical, leather, textile, cosmetic, and paper industries.