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Directed Evolution of Enantioconvergency: The Case of an Epoxide Hydrolase‐Catalyzed Reaction of a Racemic Epoxide
Author(s) -
Zheng Huabao,
Kahakeaw Daniel,
Acevedo Juan Pablo,
Reetz Manfred T.
Publication year - 2010
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.201000122
Subject(s) - epoxide hydrolase , epoxide , enantiomer , aspergillus niger , enantiomeric excess , chemistry , kinetic resolution , biocatalysis , catalysis , stereochemistry , organic chemistry , enantioselective synthesis , enzyme , biochemistry , reaction mechanism , microsome
Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 % conversion and 99 % enantiomeric excess.