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Improved Fitness of Arabidopsis thaliana Nitrilase 2
Author(s) -
Schreiner Ulrike,
Steinkellner Georg,
Rozzell J. David,
Glieder Anton,
Winkler Margit
Publication year - 2010
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.200900212
Subject(s) - nitrilase , arabidopsis thaliana , arabidopsis , chemistry , substrate (aquarium) , botany , biology , biochemistry , enzyme , gene , mutant , ecology
A matter of fitness: An Arabidopsis thaliana nitrilase 2 (NIT2) variant with improved specific activity is generated by error prone PCR followed by recombination of advantageous mutations. The improvement is shown not only for the test substrate phenylacetonitrile but also for a diverse set of aromatic nitriles, dicyanides, and other functionalized nitriles.