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A new colorimetric method  to determine the enantiopurity of chiral alcohols is presented based on enantioselective oxidation by two alcohol dehydrogenases. The oxidation of either ( R )‐ or ( S )‐alcohol produces NADPH (or NADH), which reduces 2‐(4‐iodophenyl)‐3‐(4‐ nitrophenyl)‐5‐phenyl‐2 H ‐tetrazolium to its corresponding formazan derivative. Application of this method to screen lipase variant libraries for enantioselective hydrolysis is also described.