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A High‐Throughput Screening Method for Chiral Alcohols and its Application to Determine Enantioselectivity of Lipases and Esterases
Author(s) -
BustosJaimes Ismael,
Hummel Werner,
Eggert Thorsten,
Bogo Eliane,
Puls Michael,
Weckbecker Andrea,
Jaeger KarlErich
Publication year - 2009
Publication title -
chemcatchem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.497
H-Index - 106
eISSN - 1867-3899
pISSN - 1867-3880
DOI - 10.1002/cctc.200900190
Subject(s) - enantioselective synthesis , chemistry , alcohol , lipase , hydrolysis , formazan , organic chemistry , high throughput screening , alcohol dehydrogenase , derivative (finance) , biocatalysis , alcohol oxidation , catalysis , combinatorial chemistry , enzyme , biochemistry , reaction mechanism , financial economics , economics
A new colorimetric method to determine the enantiopurity of chiral alcohols is presented based on enantioselective oxidation by two alcohol dehydrogenases. The oxidation of either ( R )‐ or ( S )‐alcohol produces NADPH (or NADH), which reduces 2‐(4‐iodophenyl)‐3‐(4‐ nitrophenyl)‐5‐phenyl‐2 H ‐tetrazolium to its corresponding formazan derivative. Application of this method to screen lipase variant libraries for enantioselective hydrolysis is also described.