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Characterization of phenolic compound antioxidant activity in oat varieties using UHPLC–online ABTS and LC Q‐TOF
Author(s) -
Rao Shiwangni,
Santhakumar Abishek B.,
Chinkwo Kenneth A.,
Blanchard Christopher L.
Publication year - 2019
Publication title -
cereal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 100
eISSN - 1943-3638
pISSN - 0009-0352
DOI - 10.1002/cche.10200
Subject(s) - chemistry , antioxidant , abts , caffeic acid , food science , polyphenol , phenols , phenolic acid , biochemistry , dpph
Background and objectives Phenolic compounds present in oats provide an opportunity for oats to increase its use in the form of functional food. In this study, eight varieties of Australian‐grown whole‐grain oat were investigated for their phenolic composition and antioxidant potential. Ultra‐high‐performance liquid chromatography–online 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid), coupled with liquid chromatography quadrupole time‐of‐flight, was used to identify and quantify individual phenolic compounds and their respective antioxidant activity. Findings Considerable variation in phenolic content (19.51 ± 1.20–52.49 ± 1.70 mg 100 g −1 GAE) and antioxidant activity was observed among the oat varieties. Several avenanthramides (AVNs) were detected with AVN A and AVN C being the most abundant. AVN C exhibited the highest antioxidant activity followed by caffeic acid, quercetin‐3,4′‐O‐di‐beta‐glucopyranoside derivative, and 6‐hydroxykaempferol 3,5,7,4′‐tetramethyl ether 6‐rhamnoside. Antioxidant activity in AVNs was observed to be the highest in AVN C followed by AVN B and AVN A. Conclusions High levels of AVN C and the range of phenolic compounds present show promise of Australian‐grown oats as an important source of antioxidant compounds. These antioxidant compounds may contribute to consumers' health benefits. Significance and novelty This study contributes to the use of oats as potential functional food by characterizing its individual phenolic compounds and their antioxidant activity.