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Three‐dimensional and quantitative analysis of atherosclerotic plaque composition by automated differential echogenicity
Author(s) -
Bruining Nico,
Verheye Stefan,
Knaapen Michiel,
Somers Pamela,
Roelandt Jos R.T.C.,
Regar Evelyn,
Heller Iddo,
de Winter Sebstiaan,
Ligthart Jurgen,
Van Langenhove Glenn,
de Feijter Pim J.,
Serruys Patrick W.,
Hamers Ronald
Publication year - 2007
Publication title -
catheterization and cardiovascular interventions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 116
eISSN - 1522-726X
pISSN - 1522-1946
DOI - 10.1002/ccd.21310
Subject(s) - echogenicity , medicine , intravascular ultrasound , differential diagnosis , pathology , radiology , ultrasound , adventitia , autopsy , biomedical engineering
Objective: To validate automated and quantitative three‐dimensional analysis of coronary plaque composition using intracoronary ultrasound (ICUS). Background: ICUS displays different tissue components based on their acoustic properties in 256 grey‐levels. We hypothesised that computer‐assisted image analysis (differential echogenicity) would permit automated quantification of several tissue components in atherosclerotic plaques. Methods and Results: Ten 40‐mm‐long left anterior descending specimens were excised during autopsy of which eight could be successfully imaged by ICUS. Histological sections were taken at 5 mm intervals and analyzed. Since most of the plaques were calcified and having a homogeneous appearance, one specimen with a more heterogeneous composition was further examined: at each interval of 5 mm, 15 additional sections (every 100 μm) were evaluated. Plaques were scored for echogenicity against the adventitia: brighter (hyperechogenic) or less bright (hypoechogenic). Areas of hypoechogenicity correlated with the presence of smooth muscle cells. Areas of hyperechogenicity correlated with presence of collagen, and areas of hyperechogenicity with acoustic shadowing correlated with calcium. None of these comparisons showed statistical significant differences. Conclusion: This ex vivo feasibility study shows that automated three‐dimensional differential echogenicity analysis of ICUS images allows identification of different tissue types within atherosclerotic plaques. This technology may play a role as an additional tool in longitudinal studies to trace possible changes in plaque composition. © 2007 Wiley‐Liss, Inc.