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Effects of contrast media on porcine bone marrow‐derived mononuclear cells and calf myoblast viability and secretion of VEGF and MCP‐1
Author(s) -
Baffour Richard,
Pakala Rajbabu,
Hellinga David,
Seabron Rufus,
Fournadjiev Jana,
Wolfram Roswitha,
Okubagzi Petros,
Epstein Stephen E.,
Waksman Ron
Publication year - 2004
Publication title -
catheterization and cardiovascular interventions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 116
eISSN - 1522-726X
pISSN - 1522-1946
DOI - 10.1002/ccd.20096
Subject(s) - medicine , peripheral blood mononuclear cell , bone marrow , secretion , vegf receptors , pathology , andrology , immunology , cancer research , in vitro , biochemistry , chemistry
We investigated the effect of contrast media on bone marrow‐derived cell viability, growth factor secretion, and myoblast viability. Bone marrow was exposed to contrast media, mononuclear cells were isolated, viability was assessed by Trypan blue exclusion or cultured for 4 weeks, and conditioned medium was assayed for vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein‐1 (MCP‐1). Skeletal myoblasts viability was assessed after exposing them to contrast media. In separate experiments, bone marrow or bone marrow‐derived mononuclear cells were exposed to contrast media, cultured for 40 hr, then assessed for viability. None of the contrast media tested had any effect on bone marrow‐derived cell viability. Hypaque or Hexabrix increased myoblasts viability by 8–10%. VEGF and MCP‐1 concentrations in the conditioned medium increased in a time‐related manner. These findings support the concept that for cell therapy, bone marrow cells or myoblasts may be mixed with contrast media and injected into ischemic myocardium without compromise in viability or function. Catheter Cardiovasc Interv 2004;62:476–481. © 2004 Wiley‐Liss, Inc.