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The effects of oxygen–ozone therapy on regulatory T‐cell responses in multiple sclerosis patients
Author(s) -
Tahmasebi Safa,
Qasim Maytham T.,
Krivenkova Maria V.,
Zekiy Angelina O.,
Thangavelu Lakshmi,
Aravindhan Surendar,
Izadi Morteza,
JadidiNiaragh Farhad,
Ghaebi Mahnaz,
Aslani Saeed,
AghebatMaleki Leili,
Ahmadi Majid,
Roshangar Leila
Publication year - 2021
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.11589
Subject(s) - ozone therapy , foxp3 , regulatory t cell , inflammation , medicine , multiple sclerosis , immune system , cell therapy , immunology , peripheral blood mononuclear cell , cell , reactive oxygen species , t cell , biology , pathology , il 2 receptor , in vitro , biochemistry , genetics , alternative medicine
Multiple sclerosis (MS) is a common degenerative disorder of the central nervous system. The decreased frequency and dysfunction of Treg cells cause inflammation and disease progression. Ozone autohemotherapy can be used as a potential therapeutic approach to regulate the immune system responses and inflammation in MS. For this purpose, 20 relapsing‐remitting multiple sclerosis patients were under treatment with ozone twice weekly for 6 months. The frequency of Treg cell, the expression levels of the Treg cell‐related factors ( FoxP3, IL‐10, TGF‐β, miR‐17, miR‐27 , and miR‐146A ), and the secretion levels of IL‐10 and TGF‐β were assessed. We found a significant increase in the number of Treg cells, expression levels of FoxP3 , miRNAs ( miR‐17 and miR‐27 ), IL‐10 , and TGF‐β factors in patients after oxygen–ozone (O 2 ‐O 3 ) therapy compared to before treatment. In contrast, oxygen–ozone therapy notably decreased the expression level of miR‐146a in treated patients. Interestingly, the secretion levels of both IL‐10 and TGF‐β cytokines were considerably increased in both serum and supernatant of cultured peripheral blood mononuclear cells in posttreatment condition compared to pretreatment condition. According to results, oxygen–ozone therapy raised the frequency of Treg cell and its relevant factors in treated MS patients. Oxygen–ozone therapy would contribute to improving the MS patients by elevating the Treg cell responses.