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Trehalose protects the endothelium from cadmium‐induced dysfunction
Author(s) -
Kumar Sarwareddy K.,
Prakash Tarun,
Vetriselvan Miralini,
Mani Krishna Priya
Publication year - 2021
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.11539
Subject(s) - enos , chemistry , nitric oxide , reactive oxygen species , oxidative stress , viability assay , mtt assay , microbiology and biotechnology , nitric oxide synthase , biochemistry , apoptosis , biology , organic chemistry
Abstract The objective of the present study is to identify the possible regulatory role of trehalose (Tre) against cadmium chloride (CdCl 2 )‐induced endothelial cell dysfunction. To screen the dose‐dependent effect of both Tre and CdCl 2 , a methylthiazolyldiphenyl‐tetrazolium bromide (MTT) assay was performed. Interestingly, MTT assay results have shown that co‐incubation of Tre (1 mM) with CdCl 2 significantly decreased the CdCl 2 (5 µM) cytotoxicity. Nitric oxide (NO) measurement using Griess assay and 4‐amino‐5‐methylamino‐2ʹ,7ʹ‐difluorofluorescein fluorescence probe results have shown that CdCl 2 decreases NO production in endothelial cells. Western blotting analysis results showed that CdCl 2 decreases endothelial nitric oxide synthase (eNOS) and phospho endothelial nitric oxide synthase (peNOS) expression. The present study results have also observed that CdCl 2 treatment increases reactive oxygen species (ROS) production. However, combination treatment (Tre + CdCl 2 ) could restore the NO production in CdCl 2 ‐treated cells. In addition, combination treatment could also restore eNOS and peNOS expression in endothelial cells. Moreover, Tre treatment was found to decrease CdCl 2 ‐induced ROS production. Collectively, the present study results demonstrate that Tre possesses a significant protective action against CdCl 2 ‐mediated endothelial dysfunction by increasing NO production, eNOS and peNOS expression, and by decreasing oxidative stress.