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Hypoxia‐induced SPOP attenuates the mobility of trophoblast cells through inhibition of the PI3K/AKT/GSK3β pathway
Author(s) -
Yuan Dong,
Yang Zhu,
Chen Yiyu,
Li Siyuan,
Tan Benxu,
Yu Qiubo
Publication year - 2021
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.11501
Subject(s) - pi3k/akt/mtor pathway , trophoblast , protein kinase b , hypoxia (environmental) , microbiology and biotechnology , chemistry , spiral artery , cytotrophoblast , ubiquitin ligase , biology , placenta , signal transduction , ubiquitin , pregnancy , biochemistry , fetus , genetics , organic chemistry , oxygen , gene
Placental hypoxia has been implicated in pregnancy pathologies such as pre‐eclampsia and intrauterine growth restriction. However, the underlying mechanism by which the trophoblasts respond to hypoxia remains unclear. Speckle‑type POZ protein (SPOP), an E3 ubiquitin ligase adapter, was previously reported to play important roles in various physiological and pathological processes. This study aims to investigate the expression and biological functions of SPOP after exposure to cobalt chloride (CoCl 2 )‐mimicked hypoxia conditions using human trophoblast‐derived choriocarcinoma cell lines and extravillous cytotrophoblast. These data showed that SPOP protein was directly induced by CoCl 2 ‐mimicked hypoxia and regulated by HIF‐1α at the posttranscription level. CoCl 2 treatment could dramatically influence the localization of SPOP in trophoblasts, especially the accumulation of SPOP into the nucleus. In addition, both CoCl 2 ‐mimicked hypoxia and induction of endogenous SPOP expression by lentivirus transfection attenuated the migration and invasion abilities of trophoblasts. Furthermore, we demonstrated that SPOP was involved in CoCl 2 ‐induced the inhibition of the PI3K/AKT/GSK3β pathway in placental trophoblasts. Taken together, these data indicate that accumulation of HIF‐1α augments the expression of SPOP in trophoblasts, which impairs trophoblastic mobility by targeting the PI3K/AKT/GSK3β pathway. This potentially leads to insufficient uterine spiral artery remodeling and suboptimal placental perfusion, and thus the development of pregnancy‐related complication.