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Long noncoding RNA SNHG12 promotes vascular smooth muscle cell proliferation and migration via regulating miR‐199a‐5p/HIF‐1α
Author(s) -
Sun Yao,
Zhao JinTao,
Chi BaoJin,
Wang Kaifeng
Publication year - 2020
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.11365
Subject(s) - gene knockdown , vascular smooth muscle , terminal deoxynucleotidyl transferase , microrna , western blot , cell growth , long non coding rna , microbiology and biotechnology , biology , apoptosis , messenger rna , transcription factor , tunel assay , rna , gene , endocrinology , biochemistry , smooth muscle
Abstract The dysregulation of proliferation and migration of vascular smooth muscle cells (VSMCs) contributes to atherosclerosis (AS) and accumulating reports indicate the crucial role of long noncoding RNA in AS. However, the role of small nucleolar RNA host gene 12 (SNHG12) in regulating the phenotypes of VSMCs and AS remains largely unknown. Quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR) was used to detect the expression levels of SNHG12 and miR‐199a‐5p in an in vivo AS model and VSMCs treated by oxidized low‐density lipoprotein (ox‐LDL). The proliferation ability, migration ability, and apoptosis of VSMCs were tested by cell counting kit‐8, Transwell assay, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay, respectively. StarBase database was used to predict the binding sites between miR‐199a‐5p and SNHG12. The interaction between miR‐199a‐5p and SNHG12 was validated by qRT‐PCR, western blot, and luciferase reporter assay. Western blot was used to examine the effects of SNHG12 and miR‐199a‐5p on the expression of hypoxia‐inducible factor 1α (HIF‐1α). We found that the expression level of SNHG12 was significantly increased in the animal model and VSMCs treated by ox‐LDL. Knockdown of SNHG12 suppressed the proliferation and migration abilities of VSMCs, while overexpression of SNHG12 had the opposite effects. Mechanically, we validated that miR‐199a‐5p was a target of SNHG12, and the target gene of miR‐199a‐5p, HIF‐1α could be indirectly and positively regulated by SNHG12. In conclusion, SHNG12 targeting miR‐199a‐5p/HIF‐1α contributed to the pathophysiological process of AS by regulating the phenotypes of VSMCs, and could be a potential therapy target for this disease.