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Trolox‐induced cardiac differentiation is mediated by the inhibition of Wnt/β‐catenin signaling in human embryonic stem cells
Author(s) -
Choe Mu Seog,
Yeo Han Cheol,
Bae Chang Min,
Han Ho Jae,
Baek Kyung Min,
Kim Joong Sun,
Lim Kyung Seob,
Shin InSik,
Chang Woochul,
Yun Seung Pil,
Lee Ho Jin,
Lee Min Young
Publication year - 2019
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.11200
Subject(s) - wnt signaling pathway , trolox , embryonic stem cell , microbiology and biotechnology , induced pluripotent stem cell , stem cell , biology , embryoid body , cellular differentiation , directed differentiation , dkk1 , chemistry , signal transduction , biochemistry , gene , antioxidant , dpph
Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β‐catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6‐Hydroxy‐2,5,7,8‐tetramethylchromane‐2‐carboxylic acid (Trolox) significantly enhanced cardiac differentiation in a time‐ and dose‐dependent manner after the mesodermal differentiation of hESCs. Trolox promoted hESC cardiac differentiation through its inhibitory activity against the Wnt/β‐catenin pathway. This study demonstrates an efficient cardiac differentiation method and reveals a novel Wnt/β‐catenin regulator.