Premium
The influence of serum‐supplemented culture media in a transwell migration assay
Author(s) -
Omar Zaki Siti Sarah,
Kanesan Livashini,
Leong Ming Yeh Danielle,
Vidyadaran Sharmili
Publication year - 2019
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.11122
Subject(s) - chemotaxis , cell migration , microglia , chemistry , fetal bovine serum , microbiology and biotechnology , cell culture , chemotaxis assay , cell , immunology , biology , biochemistry , inflammation , genetics , receptor
Our work cautions against the use of serum‐supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS‐supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.