The influence of serum‐supplemented culture media in a transwell migration assay | Zendy

Omar Zaki Siti Sarah | Zendy; Kanesan Livashini | Zendy; Leong Ming Yeh Danielle | Zendy; Vidyadaran Sharmili | Zendy

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The influence of serum‐supplemented culture media in a transwell migration assay

Author(s) -

Omar Zaki Siti Sarah,

Kanesan Livashini,

Leong Ming Yeh Danielle,

Vidyadaran Sharmili

Publication year - 2019

Publication title -

cell biology international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.932

H-Index - 77

eISSN - 1095-8355

pISSN - 1065-6995

DOI - 10.1002/cbin.11122

Subject(s) - chemotaxis , cell migration , microglia , chemistry , fetal bovine serum , microbiology and biotechnology , cell culture , chemotaxis assay , cell , immunology , biology , biochemistry , inflammation , genetics , receptor

Our work cautions against the use of serum‐supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS‐supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.

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