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Multidrug resistance phenotype: Relation between phenotype induction and its characteristics in erythroleukemia cells
Author(s) -
Soares Fernanda Saldanha,
Lettnin Aline Portantiolo,
Wagner Eduardo Felipe,
Mattozo Francielly Hafele,
CarrettDias Michele,
Rumjanek Vivian Mary Barral Dodd,
Filgueira Daza Moraes Vaz Batista,
de Souza Votto Ana Paula
Publication year - 2019
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.11095
Subject(s) - cytotoxicity , multiple drug resistance , phenotype , k562 cells , cell culture , apoptosis , cell , biology , cancer cell , dna damage , cancer research , microbiology and biotechnology , in vitro , drug resistance , dna , cancer , gene , biochemistry , genetics
Chemotherapy may be followed by multiple drug resistance (MDR). This is an obstacle in the treatment of cancer. It is therefore essential to understand the mechanisms underlying tumor resistance, especially those involved in the cell target/MDR relationship. To investigate this, the effects of exposing cells to UVB (to target DNA), UVA, and H 2 O 2 (to target the cell membrane) were observed in K562 (non MDR) and FEPS (MDR) cell lines. The K562 cells were more sensitive to UVA than the FEPS cells. The FEPS cell line was more resistant to H 2 O 2 than K562, only presenting cytotoxicity 72 h after being exposed to 40 mM, with no ROS increase until 48 h. Both cell lines were sensitive to UVB, presenting cytotoxicity after 24 h, mainly by apoptosis, and showed an increase in ROS levels. Our results indicate that agents acting on DNA may be able to overcome the MDR phenotype.