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IL‐7 enhances the differentiation of adipose‐derived stem cells toward lymphatic endothelial cells through AKT signaling
Author(s) -
Sun Yiyu,
Lu Bolun,
Deng Jingcheng,
Jiang Zhaohua,
Cao Weigang,
Dai Tingting,
Li Shengli
Publication year - 2019
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.11093
Subject(s) - lymphatic endothelium , protein kinase b , pi3k/akt/mtor pathway , microbiology and biotechnology , flow cytometry , mapk/erk pathway , stem cell , adipose tissue , chemistry , phosphorylation , biology , lymphatic system , signal transduction , immunology , endocrinology
Abstract Our study was designed to investigate the effects of IL‐7 during the differentiation process of adipose‐derived stem cells (ADSCs) toward lymphatic endothelial cells (LECs). IL‐7 was added to the traditional induced medium, which was called the IL‐7 (+) group, while the group that used traditional induced medium was called the IL‐7 (−) group. After 7 days of induction of ADSCs, a comprehensive analysis was conducted between these two groups. We examined the changes in Prox1, LYVE‐1, Podoplanin and VEGFR‐3 on the RNA and protein level and found that the expression of LEC markers in the IL‐7 (+) group was higher than in the IL‐7 (−) group. The characteristics of differentiated cells were confirmed by flow cytometry and immunofluorescence. At the same time, we detected the MAPK/ERK and PI3K/AKT pathway involved in the differentiation process, and we found that the phosphorylation of AKT increased, however the expression of ERK was not significantly changed. In conclusion, our study found that IL‐7 could improve the differentiation efficiency of ADSCs toward LECs through AKT signaling pathways.