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MicroRNA‐1254 contributes to the controlling of pro‐fibrogenic environment in LX‐2 cells by modulating SMAD3 and wound repair: new insights in hepatic fibrosis
Author(s) -
Gonçalves Letícia Rocha,
de Oliveira da Silva Brenda,
Scarinci Letícia Destefani,
Ramos Letícia Ferreira,
Moraes Karen C.M.
Publication year - 2019
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.11075
Subject(s) - hepatic stellate cell , hepatic fibrosis , microrna , cirrhosis , wound healing , fibrosis , cancer research , steatosis , biology , microbiology and biotechnology , pi3k/akt/mtor pathway , cellular model , angiotensin ii , protein kinase b , signal transduction , medicine , cell culture , gene , endocrinology , immunology , receptor , biochemistry , genetics
Hepatic fibrosis and its end‐stage cirrhosis have increased worldwide, and, despite all the efforts, no successful therapy is available. More recently, the heptapeptide angiotensin‐(1‐7) [ang‐(1‐7)] was reported to be able to modulate liver fibrosis and even steatosis; however, the molecular bases of these effects are not clear. In this study, we investigated the overexpression of the microRNA‐1254 in the human hepatic stellate cell line LX‐2, based on the effect of the heptapeptide in such cells, previously, demonstrated by our research group. In addition, this miRNA was chosen based on the identification of putative binding site of this small molecule in the mRNA sequences of different molecular connectors of the AKT/ PI3K pathway, which is modulated by the heptapeptide and connects to the control of several cellular mechanisms, including proliferation, survival, migration, and even liver fibrogenesis. The results revealed an innovative function of the miR‐1254 in controlling SMAD3 and pro‐fibrosing elements as well as the wound healing response in LX‐2, attenuating the scaring repair of the injured tissue. The combined findings provide useful information for future studies on the controlling of hepatic fibrogenesis.