Premium
Effects of VEGF‐ANG‐1‐PLA nano‐sustained release microspheres on proliferation and differentiation of ADSCs
Author(s) -
He Yucang,
Li Zihao,
Chen Zhuojie,
Yu Xiaofang,
Ji Ziwan,
Wang Jingping,
Qian Yao,
Li Liqun
Publication year - 2018
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10986
Subject(s) - vascular endothelial growth factor , angiogenesis , stromal cell , chemistry , polylactic acid , neovascularization , microbiology and biotechnology , vegf receptors , cancer research , medicine , biology , organic chemistry , polymer
The improvement of fat graft viability might depend on the presence of multipotent resident adipose derived stem cells (ADSCs) which is the important component of stromal vascular fraction (SVF). Vascular endothelial growth factor (VEGF) and angiogenin‐1 (Ang‐1) are responsible for neovascularization. However, their half‐life is too short to produce a biological effect. We thus investigated whether VEGF‐ANG‐1‐polylactic acid (PLA) microspheres could enhance the angiogenic properties of ADSCs. PLA microspheres containing VEGF and ANG‐1 were prepared by in vitro ultrasonic emulsification and characterized according to their encapsulation efficiency (EE), drug‐loading rate (DL), particle size, and drug release. The systemic toxicity of empty loaded nanospheres (NPs) and the ability of these microspheres to promote the proliferation and differentiation of ADSCs were evaluated. The EE and DL were above 86 and 2.8%, respectively. The drug release was completed after 20 days. Systemic toxicity was verified in ADSCs that received the unloaded NPs. It was observed that ADSCs treated with VEGF‐ANG‐1‐PLA microspheres had an increase in the proliferation and the number of CD31 positive cells. ADSCs proliferation and differentiation toward endothelial cells (ECs) could be enhanced by the addition of VEGF‐ANG‐1‐PLA nano‐sustained release microspheres.