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Myricetin‐induced oxidative stress suppresses murine T lymphocyte activation
Author(s) -
GhassemiRad Javad,
Maleki Mahdis,
Knickle Allison F.,
Hoskin David W.
Publication year - 2018
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10977
Subject(s) - myricetin , oxidative stress , t cell , chemistry , cytotoxic t cell , immune system , reactive oxygen species , antigen presenting cell , microbiology and biotechnology , biology , biochemistry , antioxidant , immunology , flavonoid , in vitro , kaempferol
A number of polyphenolic compounds present in fruits and vegetables have the capacity to modulate immune responses; however, the impact of the common plant‐derived flavonoid myricetin on T lymphocyte function has not been investigated. We show that myricetin inhibited mouse T lymphocyte activation by bead‐immobilized anti‐CD3 and anti‐CD28 monoclonal antibodies, as indicated by a dose‐dependent reduction in cell proliferation and decreased synthesis of interferon‐γ, interleukin (IL)‐2, IL‐4, and IL‐17 associated with different T helper cell subsets. This effect was attributed to myricetin‐induced reactive oxygen species (ROS) since myricetin caused hydrogen peroxide (H 2 O 2 ) to accumulate in cell‐free culture medium and H 2 O 2 inhibited T cell proliferation and cytokine synthesis. In addition, the antioxidant N‐acetyl cysteine restored the ability of myricetin‐treated T lymphocytes to proliferate in response to a mitogenic stimulus. The presence of dendritic cells or bone marrow‐derived macrophages negated the inhibitory effect of myricetin on T cell activation, and H 2 O 2 in T cell cultures that were treated with exogenous H 2 O 2 was reduced when antigen‐presenting cells were also present. These findings suggest that antioxidant molecules produced by dendritic cells and macrophages protected T cells from myricetin‐induced oxidative stress, and underscore the importance of considering immune cell interactions when evaluating the immunomodulatory activity of ROS‐generating phytochemicals.

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