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Hypoxia induces apoptosis of mouse spermatocyte GC‐2 cells through activation of autophagy
Author(s) -
Zhou Jian,
Qian ChunYa,
Tong RuiQing,
Wang Bin,
Chen XueLei,
Zhuang YanYan,
Xia Fei,
He Qi,
Lv JinXing
Publication year - 2018
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10971
Subject(s) - apoptosis , autophagy , microbiology and biotechnology , flow cytometry , hypoxia (environmental) , tunel assay , biology , programmed cell death , chemistry , biochemistry , organic chemistry , oxygen
The aim of this study was to investigate the underlying mechanisms of hypoxia‐induced apoptosis of GC‐2spd (GC‐2) cells. The GC‐2 cells were treated with or without hypoxia for 12, 24, 48, and 72 h. Apoptosis of GC‐2 cells was detected using TUNEL and flow cytometry. Fluorescence microscopic was used to observe the autophagy of GC‐2 cells. Hypoxia‐inducible factor‐1alpha (HIF‐1α), apoptosis‐related protein and marker protein of autophagy levels were measured by Western blotting. Hypoxia induced apoptosis and autophagy of GC‐2 cells, and increased expression of HIF‐1α, LC3‐II, Beclin‐1, and pro‐apoptotic protein, but reduced p62 and anti‐apoptotic protein level. Meanwhile, hypoxia‐induced HIF‐1α mediated expression of apoptosis and autophagy‐related protein in GC‐2 cell. Furthermore, autophagy regulated hypoxia‐induced apoptosis of GC‐2 cell. Our data suggest that hypoxia induces apoptosis of GC‐2 cell by activation of autophagy involving activation of the apoptosis signaling pathway under the hypoxic condition.