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Mitochondrial ATAD3A regulates milk biosynthesis and proliferation of mammary epithelial cells from dairy cow via the mTOR pathway
Author(s) -
Chen Dongying,
Yuan Xiaohan,
Liu Lijie,
Zhang Minghui,
Qu Bo,
Zhen Zhen,
Gao Xuejun
Publication year - 2018
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10929
Subject(s) - pi3k/akt/mtor pathway , microbiology and biotechnology , cell growth , cyclin d1 , rptor , biology , phosphorylation , biosynthesis , protein kinase b , signal transduction , cell , chemistry , cell cycle , biochemistry , enzyme
ATPase family AAA‐domain containing protein 3A (ATAD3A) is a nuclear‐encoded mitochondrial membrane protein, which is essential for cell growth and metabolism. The mechanism by which ATAD3A acts is still not fully understood. In this study, we explored the regulatory role of ATAD3A on milk biosynthesis and proliferation of bovine mammary epithelial cell. We showed that ATAD3A is localized in mitochondria and the expression of ATAD3A was up‐regulated in response to extracellular stimuli such as amino acids and hormones. We observed that ATAD3A positively regulated milk protein, fat, and lactose biosynthesis, and cell proliferation. We further revealed that ATAD3A promoted the expressions of mTOR, SREBP‐1c, and Cyclin D1, and triggers mTOR phosphorylation. In summary, our data reveal that ATAD3A regulates the mTOR, SREBP‐1c, and Cyclin D1 signaling pathways for milk biosynthesis and cell proliferation.