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Comparative study of hematopoietic stem and progenitor cells between sexes in mice under physiological conditions along time
Author(s) -
Gasco Samanta,
Rando Amaya,
Zaragoza Pilar,
GarcíaRedondo Alberto,
Calvo Ana Cristina,
Osta Rosario
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10865
Subject(s) - haematopoiesis , progenitor cell , biology , stem cell , flow cytometry , myeloid , progenitor , immunology , microbiology and biotechnology , peripheral blood
Hematopoietic stem and progenitor cells (HSPCs) are attractive targets in regenerative medicine, although the differences in their homeostatic maintenance between sexes along time are still under debate. We accurately monitored hematopoietic stem cells (HSCs), common lymphoid progenitors (CLPs), and common myeloid progenitors (CMPs) frequencies by flow cytometry, by performing serial peripheral blood extractions from male and female B6SJL wild‐type mice and found no significant differences. Only modest differences were found in the gene expression profile of Slamf1 and Gata2 . Our findings suggest that both sexes could be used indistinctly to perform descriptive studies in the murine hematopoietic system, especially for flow cytometry studies in peripheral blood. This would allow diminishing the number of animals needed for the experimental procedures. In addition, the use of serial extractions in the same animals drastically decreases the number of animals needed.