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Cooperation of HIF‐ and NCAM‐mediated mechanisms in cell viability of hippocampal cultures after oxygen–glucose deprivation
Author(s) -
Lushnikova Iryna,
Nikandrova Yelyzaveta,
Skibo Galyna
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10825
Subject(s) - hippocampal formation , microbiology and biotechnology , viability assay , oxygen , chemistry , cell , neuroscience , biology , biochemistry , organic chemistry
Neurodegenerative diseases of different genesis are the result of cellular damages including those caused by oxygen and glucose deficit. Neuronal survival or death in brain pathologies depends on a variety of interrelated molecular mechanisms. A key role in modulation of neuron viability belongs to HIF (hypoxia‐inducible factor) and NCAM (neural cell adhesion molecules) signaling pathways. In this work, we used organotypic and dissociated hippocampal cultures to analyze cell viability and HIF‐1α immunopositive (HIF‐1α + ) signal after 30 min oxygen–glucose deprivation (OGD) followed by 24 h of reoxygenation in the presence of FGL (synthetic NCAM‐derived mimetic peptide). According to LDH‐ and MTS‐assay of cell viability, FGL showed a neuroprotective effect, which was attributed to the association with FGFR. We showed that these effects correlated with changes of the HIF‐1α + level suggesting the communications of HIF and NCAM signaling pathways. These data extend our knowledge of neurodegeneration mechanisms and open additional potential for the development of neuroprotection strategies.