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Differential expression profile of long non‐coding RNA in cardiomyocytes autophagy induced by angiotensin II
Author(s) -
Gu Ying,
Yang Fan,
Xu Ruming,
Zhang Yunyan,
Li Yang,
Liu Suxuan,
Zhang Guanxin,
Wang Guokun,
Ma Liping
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10809
Subject(s) - autophagy , downregulation and upregulation , microbiology and biotechnology , biology , microrna , angiotensin ii , microarray analysis techniques , intracellular , western blot , gene expression , genetics , gene , apoptosis , receptor
Autophagy is a ubiquitous intracellular process for cellular homeostasis maintenance by recycling damaged protein and organelles. Dysregulation of cardiomyocytes autophagic activity is implicated in various heart diseases. Recent studies had demonstrated that long non‐coding RNAs (lncRNAs) played crucial roles on modulation of autophagic activity. In this study, we first established an angiotensin II‐induced autophagy model on neonatal rat cardiomyocytes. Western blot assay confirmed that the expression of Beclin 1 and the conversion of soluble LC3‐I to lipid bound LC3‐II were significantly increased at 12 h after angiotensin II stimulation, but the cardiomyocytes surface area and hypertrophic markers expression had no significant change. Then microarray analysis and real‐time PCR were applied to detect differentially expressed lncRNAs during cardiomyocytes autophagy. A total of 1,249 lncRNAs were determined as differentially expressed, including 700 upregulated lncRNAs and 549 downregulated lncRNAs. LncRNAs subgroup analysis showed there were 43 transcribed ultra‐conserved noncoding RNAs (T‐UCRs) differentially expressed in cardiomyocytes autophagy, of which 26 T‐UCRs were upregulated and 17 T‐UCRs were downregulated. Bioinformatics analysis further showed that 94 differentially expressed lncRNAs contained potential binding sites of miR‐22, a pro‐hypertrophic and pro‐autophagic microRNA. Therefore, these differentially expressed lncRNAs might play critical roles in cardiomyocytes autophagy. This finding would provide an experimental basis for future investigation on ischemic heart disease.