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microRNA‐216b inhibits cell proliferation and migration in human melanoma by targeting FOXM1 in vitro and in vivo
Author(s) -
Sun Mengyao,
Wang Xiaopeng,
Tu Chen,
Wang Shuang,
Qu Jianqiang,
Xiao Shengxiang
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10754
Subject(s) - microrna , biology , melanoma , cell growth , cancer research , foxm1 , cell , cell cycle , gene , genetics
MicroRNAs (miRNAs) play an increasingly important role in cancer growth by coordinately suppressing genes that control cell migration, proliferation, and invasion. The above results can be achieved through the regulation of gene expression by miRNAs by suppressing translation or the direct sequence‐specific degradation of the targeted mRNA. In the present study, we indicate that the expression of miR‐216b could be effectively repressed both in human melanoma tissues through a comparison with primary melanoma and in human melanoma cell lines through a comparison with a normal human keratinocyte line. Moreover, miR‐216b induced a clear decrease in melanoma cell proliferation and migration in vitro. Forkhead box M1 (FOXM1) was confirmed as a target gene of miR‐216b, and the overexpression of miR‐216b markedly repressed the luciferase activity of reporter plasmids containing the FOXM1 3′‐UTR (untranslated region). Furthermore, miR‐216b suppressed melanoma cell growth in nude mice in vivo, with the effects of miR‐216b overexpression on melanoma cell growth and proliferation reversed by FOXM1 overexpression. The results demonstrated that miR‐216b is a tumor suppressor in melanoma, identified the FOXM1 signaling pathway as a target of miR‐216b action, and suggested a potential therapeutic role for miR‐216b in melanoma.