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Growth hormone modulates in vitro endothelial cell migration and formation of capillary‐like structures
Author(s) -
Messias de Lima Cícero Fagner,
dos Santos Reis Maria Danielma,
da Silva Ramos Fernando Wagner,
AyresMartins Silvana,
Smaniotto Salete
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10747
Subject(s) - matrigel , angiogenesis , fibronectin , extracellular matrix , laminin , microbiology and biotechnology , endothelial stem cell , growth factor , in vitro , biology , medicine , endocrinology , hormone , chemistry , cancer research , biochemistry , receptor
The generation of new blood vessels is a complex process mediated by a variety of growth factors, and the growth hormone (GH) has been shown to act as a proangiogenic factor. In fact, human GH deficiency or excess are associated with endothelial dysfunction. Moreover, mouse models have revealed the action of GH in both tissue repair and in the microvascular circulation of normal tissues. In this study, we investigated the in vitro effects of GH on endothelial cells. Using a murine endothelioma cell line (tEnd.1), we demonstrated that GH has a mitogenic effect. The hormone also affected the endothelial cellular morphology and augmented the deposition of the extracellular matrix molecules, laminin, and fibronectin, on tEnd.1 surface. GH could stimulate tEnd.1 cell fugetaxis, in transwell chambers migration assay, and increased the formation of capillary‐like structures in Matrigel®‐coated plates. Given the important role of angiogenesis during tissue injury, for example, at ischemic lesions, these findings shed light on therapeutic angiogenesis, particularly in pathologies where the cardiovascular system has been compromised.