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Propofol protects against hepatic ischemia/reperfusion injury via miR‐133a‐5p regulating the expression of MAPK6
Author(s) -
Hao Wei,
Zhao ZhiHui,
Meng QingTao,
Tie MuEr,
Lei ShaoQing,
Xia ZhongYuan
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10745
Subject(s) - propofol , reperfusion injury , pharmacology , ischemia , medicine , cardiology
Propofol has been found to play an important role in hepatic ischemia/reperfusion (I/R) injury with the antioxidant effects. However, the molecular mechanism of propofol in hepatic I/R injury has not been fully understood. Male Sprague–Dawley rats were randomly assigned into Sham group, hepatic I/R group, and propofol treatment group. I/R injury was attained by ischemia for 1 h and reperfusion for 2 h. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity were detected. QSG‐7701 cells were cultured in hypoxia condition for 15 h and then in reoxygenation condition for 6 h to imitate hypoxia/reoxygenation (H/R) injury in vitro. Real‐time RT‐PCR and Western blot were performed to determine the expression of miR‐133a‐5p and MAPK6. Luciferase reporter assay was used to determine the regulation of miR‐133a‐5p on MAPK6. Propofol significantly reduced the activities of serum AST and ALT induced by hepatic I/R injury in rats. Propofol increased the level of miR‐133a‐5p and decreased the expression of MAPK6 in vivo and in vitro. Luciferase reporter assay showed that MAPK6 was a target of miR‐133a‐5p. Knockdown of miR‐133a‐5p abrogated the effect of propofol on the upregulation of MAPK6 induced by H/R. MAPK6 overexpression promoted the cell apoptosis induced by H/R which could be attenuated by propofol. Finally, we found that miR‐133a‐5p reversed the protective effect of propofol in rats with hepatic I/R injury. Propofol showed protective roles for hepatic I/R injury in vivo and H/R injury in vitro, which involved with miR‐133a‐5p regulating the expression of MAPK6.