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Influence of a critical single nucleotide polymorphism on nuclear receptor PXR‐promoter function
Author(s) -
Rana Manjul,
Coshic Poonam,
Goswami Ravinder,
Tyagi Rakesh K.
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10744
Subject(s) - pregnane x receptor , biology , nuclear receptor , single nucleotide polymorphism , allele , gene , transcription factor , microbiology and biotechnology , reporter gene , promoter , transcriptional regulation , genetics , receptor , gene expression , regulation of gene expression , xenobiotic , genotype , biochemistry , enzyme
The Pregnane and Xenobiotic Receptor (PXR; NR1I2) is a ligand‐modulated transcription factor that belongs to the nuclear receptor superfamily. It is expressed at higher levels primarily in liver and intestine as compared to the levels in several other organs. It is activated by a broad spectrum of xenobiotics and endobiotics. The primary function of PXR is to regulate the expression of drug metabolizing enzymes and transporters and prevent the accumulation of toxic chemicals in the body, thereby maintaining body's homeostasis. In this study, we identified a C/T single nucleotide polymorphism at position −831 from the transcriptional start site of the PXR gene promoter and examined the functional significance of this variant using both the luciferase reporter gene assays and electrophoretic mobility shift assays (EMSA). Transient transfection experiments showed that the T‐allele was associated with significantly greater transcriptional activity than the C‐allele of SNP rs3814055. These results indicate that the −831C/T polymorphism has a direct effect on transcriptional regulation of PXR gene. This allelic variation may be a potential genetic marker that can help identify individuals at higher risk for Inflammatory Bowel Disease (IBD).