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Salmonella effector SopD2 interferes with Rab34 function
Author(s) -
Teo Wei Xuan,
Yang Zhe,
Kerr Markus Charles,
Luo Lin,
Guo Zhong,
Alexandrov Kirill,
Stow Jenny Lea,
Teasdale Rohan David
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10739
Subject(s) - rab , salmonella enterica , effector , biology , salmonella , intracellular parasite , intracellular , pathogenicity island , microbiology and biotechnology , virulence , secretion , gtpase , vacuole , type three secretion system , bacteria , cytoplasm , genetics , gene , biochemistry
Many intracellular pathogens have evolved highly specialized mechanisms to isolate themselves from the host cell's innate immune response while still obtaining the necessary nutrients to survive. Salmonella utilizes type 3 secretion systems (T3SSs) to deliver bacterial proteins called effectors, across the encompassing Salmonella Containing vacuole (SCV) membrane, to subvert the host's membrane trafficking pathways and alter other cellular processes. The Salmonella Pathogenicity Island (SPI)‐2 effector SopD2 has recently been demonstrated to modulate multiple members of the Rab GTPase family such as Rab7, Rab8, Rab10, and Rab32 (D'Costa et al., [D'Costa VM, 2015], Cell Reports, 12:1508–18; Spano et al., [Spano S, 2016], Cell Host & Microbe, 19:216–26). Here, we demonstrate the additional capacity of SopD2 to bind Rab34 and modulate its function. Our data indicate that depletion of Rab34 delays maturation of the SCV, and consequently, inhibits intracellular Salmonella enterica serotype typhimurium ( S. typhimurium ) growth. Interestingly, intracellular growth of the S. typhimurium lacking SopD2 was severely impaired in Rab34‐depleted cells, suggesting a compounding virulence effect. Overall this study reveals an additional member of the Rab GTPase family, Rab34, that is modulated by SopD2 and provides insight into its role in Salmonella biology.