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MicroRNA‐195‐5p suppresses osteosarcoma cell proliferation and invasion by suppressing naked cuticle homolog 1
Author(s) -
Qu Qiang,
Chu Xiangdong,
Wang Peng
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10723
Subject(s) - osteosarcoma , microrna , transfection , cancer research , cell growth , gene silencing , chemistry , luciferase , cell culture , reporter gene , cell , microbiology and biotechnology , biology , gene expression , gene , biochemistry , genetics
MiR‐195‐5p has been shown to play an essential role in human cancer progression. Nevertheless, the biological role of miR‐195‐5p in osteosarcoma development remains unclear. In this study, real‐time PCR was performed to examine the miR‐195‐5p expression in human osteosarcoma cell lines. CCK‐8 assay and Transwell assay were carried out to measure the effect of miR‐195‐5p on cell proliferation and invasion. Luciferase reporter assay was used to identify the targets of miR‐195‐5p. The results showed that miR‐195‐5p was significantly downregulated in osteosarcoma cells. Forced expression of miR‐195‐5p significantly inhibited cell proliferation, suppressed cell migration and invasion, compared with wild‐type and control‐transfected osteosarcoma cells. Luciferase reporter assay revealed that miR‐195‐5p binds to the 3′‐untranslated region (UTR) of Naked cuticle homolog 1 (NKD1), indicating that NKD1 was a novel target of miR‐195‐5p. NKD1 mRNA and protein levels were reduced after overexpression of miR‐195‐5p. Moreover, silencing of NKD1 significantly inhibited the proliferation and invasion of osteosarcoma cells. Accordingly, our results support a tumor suppressor role of miR‐195‐5p in osteosarcoma through inhibiting NKD1, and it may be a promising therapeutic target for osteosarcoma.