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Adrenaline inhibits osteogenesis via repressing miR‐21 expression
Author(s) -
Chen Danying,
Wang Zuolin
Publication year - 2017
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10685
Subject(s) - runx2 , chromatin immunoprecipitation , microbiology and biotechnology , chemistry , microrna , cellular differentiation , acetylation , histone , regulation of gene expression , endocrinology , gene expression , biology , biochemistry , gene , promoter
Abstract Sympathetic signaling is involved in bone homeostasis; however, the cellular and molecular mechanisms remain unknown. In this study, we found that the psychological stress mediator adrenaline inhibited osteogenic differentiation of human bone marrow‐derived stem cells (hMSC) by reducing microRNA‐21 (miR‐21) expression. Briefly, adrenaline significantly inhibited the osteogenic differentiation of hMSCs, as observed with both Alizarin red staining and maker gene expression (RUNX2, OSX, OCN, and OPN). During this process, miR‐21 was suppressed by adrenaline via inhibition of histone acetylation, as verified by H3K9Ac chromatin immunoprecipitation (ChIP) assay. MiR‐21 was confirmed to promote hMSC osteogenic differentiation, and overexpression of miR‐21 reversed the impeditive effect of adrenaline on hMSC osteogenic differentiation. Our results demonstrate that down‐regulation of miR‐21 is responsible for the adrenaline‐mediated inhibition of hMSC osteogenic differentiation. These findings indicate a regulation of bone metabolism by psychological stress and also provide a molecular basis for psychological stress‐associated bone diseases.