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Sphingosine‐1‐phosphate‐enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells
Author(s) -
Hashimoto Yoko,
Kobayashi Mari,
Matsuzaki Etsuko,
Higashi Katsumasa,
TakahashiYanaga Fumi,
Takano Aiko,
Hirata Masato,
Nishimura Fusanori
Publication year - 2016
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10652
Subject(s) - wnt5a , microbiology and biotechnology , alkaline phosphatase , wnt signaling pathway , sphingosine 1 phosphate , chemistry , cellular differentiation , lrp5 , signal transduction , sphingosine , receptor , biology , biochemistry , enzyme , gene
In this study, we investigated the involvement of Wnt signaling in sphingosine‐1‐phosphate (S1P)‐enhanced osteogenic differentiation of C3H10T1/2 pluripotent stem cells. We found that S1P enhanced the expression of Wnt5a and low‐density lipoprotein receptor‐related protein 5 or 6 (LRP5/6) during osteogenic differentiation. Wnt5a‐neutralizing antibody inhibited S1P‐enhanced expression of LRP5/6 and alkaline phosphatase, which are essential for osteogenic differentiation. Conversely, S1P did not affect endogenous canonical Wnt signaling. Taken together, S1P‐enhanced Wnt5a promotes LRP5/6 expression, resulting in the trigger of osteogenic differentiation of C3H10T1/2 cells. These findings suggest a potential beneficial role for S1P in bone regeneration.