Sphingosine‐1‐phosphate‐enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells | Zendy

Hashimoto Yoko | Zendy; Kobayashi Mari | Zendy; Matsuzaki Etsuko | Zendy; Higashi Katsumasa | Zendy; TakahashiYanaga Fumi | Zendy; Takano Aiko | Zendy; Hirata Masato | Zendy; Nishimura Fusanori | Zendy

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Sphingosine‐1‐phosphate‐enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2 cells

Author(s) -

Hashimoto Yoko,

Kobayashi Mari,

Matsuzaki Etsuko,

Higashi Katsumasa,

TakahashiYanaga Fumi,

Takano Aiko,

Hirata Masato,

Nishimura Fusanori

Publication year - 2016

Publication title -

cell biology international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.932

H-Index - 77

eISSN - 1095-8355

pISSN - 1065-6995

DOI - 10.1002/cbin.10652

Subject(s) - wnt5a , microbiology and biotechnology , alkaline phosphatase , wnt signaling pathway , sphingosine 1 phosphate , chemistry , cellular differentiation , lrp5 , signal transduction , sphingosine , receptor , biology , biochemistry , enzyme , gene

In this study, we investigated the involvement of Wnt signaling in sphingosine‐1‐phosphate (S1P)‐enhanced osteogenic differentiation of C3H10T1/2 pluripotent stem cells. We found that S1P enhanced the expression of Wnt5a and low‐density lipoprotein receptor‐related protein 5 or 6 (LRP5/6) during osteogenic differentiation. Wnt5a‐neutralizing antibody inhibited S1P‐enhanced expression of LRP5/6 and alkaline phosphatase, which are essential for osteogenic differentiation. Conversely, S1P did not affect endogenous canonical Wnt signaling. Taken together, S1P‐enhanced Wnt5a promotes LRP5/6 expression, resulting in the trigger of osteogenic differentiation of C3H10T1/2 cells. These findings suggest a potential beneficial role for S1P in bone regeneration.

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