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Proliferation of mouse fibroblast‐like and osteoblast‐like cells on pure titanium films manufactured by electron beam melting
Author(s) -
Kawase Mayu,
Hayashi Tatsuhide,
Asakura Masaki,
Tomino Masafumi,
Mieki Akimichi,
Kawai Tatsushi
Publication year - 2016
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10642
Subject(s) - biocompatibility , osteoblast , titanium , biomaterial , fibroblast , materials science , alizarin red , contact angle , biomedical engineering , biophysics , chemistry , nanotechnology , staining , chemical engineering , composite material , metallurgy , biochemistry , in vitro , biology , medicine , genetics , engineering
Abstract The physical characteristics and biological compatibility of surfaces produced by electron beam melting (EBM) are not well known. In particular, there are not many reports on biocompatibility qualities. In this study, pure Ti films were manufactured using EBM. While it is reported that moderately hydrophilic biomaterial surfaces display improved cell growth and biocompatibility, contact angle measurements on the EBM‐produced pure Ti films showed slight hydrophobicity. Nonetheless, we found the cell count of both fibroblast‐like cells (L929) and osteoblast‐like cells (MC3T3‐E1) increased on pure Ti films, especially the MC3T3‐E1, which increased more than that of the control. In addition, the morphology of L929 and MC3T3‐E1 was polygonal and spindle‐shaped and the cytoskeleton was well developed in the pure Ti surface groups. Upon staining with Alizarin red S, a slight calcium deposition was observed and this level gradually rose to a remarkable level. These results indicate that pure Ti films manufactured by EBM have good biocompatibility and could be widely applied as biomedical materials in the near future.