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Basal neutrophil function in human aging: Implications in endothelial cell adhesion
Author(s) -
NogueiraNeto Joes,
Cardoso André S. C.,
Monteiro Hugo P.,
Fonseca Fernando L. A.,
Ramos Luiz Roberto,
Junqueira Virginia B. C.,
Simon Karin A.
Publication year - 2016
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10618
Subject(s) - cd11a , cd18 , basal (medicine) , immunology , adhesion , integrin alpha m , cell adhesion molecule , intracellular , in vitro , ageing , inflammation , endothelial stem cell , cell adhesion , microbiology and biotechnology , neutrophile , biology , cell , chemistry , medicine , endocrinology , biochemistry , flow cytometry , organic chemistry , insulin
Much attention has been drawn to the pro‐inflammatory condition that accompanies aging. This study compared parameters from non‐stimulated neutrophils, obtained from young (18–30 years old [y.o.]) and elderly (65–80 y.o.) human volunteers. Measured as an inflammatory marker, plasmatic concentration of hs‐CRP was found higher in elderly individuals. Non‐stimulated neutrophil production of ROS and NO was, respectively, 38 and 29% higher for the aged group. From the adhesion molecules evaluated, only CD11b expression was elevated in neutrophils from the aged group, whereas no differences were found for CD11a, CD18, or CD62. A 69% higher non‐stimulated in vitro neutrophil/endothelial cell adhesion was observed for neutrophils isolated from elderly donors. Our results suggest that with aging, neutrophils may be constitutively producing more reactive species in closer proximity to endothelial cells of vessel walls, which may both contribute to vascular damage and reflect a neutrophil intracellular disrupted redox balance, altering neutrophil function in aging.