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Generation and characterization of bovine bone marrow‐derived macrophage cell line
Author(s) -
Xiao Jiajia,
Xie Rongxia,
Li Qiaoqiao,
Chen Wuju,
Zhang Yong
Publication year - 2016
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10599
Subject(s) - macrophage , biology , telomerase reverse transcriptase , innate immune system , immune system , cell culture , bone marrow , immunology , antigen , telomerase , microbiology and biotechnology , gene , in vitro , genetics , biochemistry
Macrophages, as the forefront of innate immune defense, have an important role in the host responses to mycobacterial infection. Therefore, a stable macrophage cell line is needed for future bovine immune system research on the bacterial infection. In this study, we established a bovine macrophage cell line by introducing the human telomerase reverse transcriptase (hTERT) gene into bovine bone marrow‐derived macrophages (bBMMs). The TERT‐bBMMs cells expressed macrophage surface antigen (CD11b, CD282) and upregulated expression of the cytokines IL‐1β, IL‐6, IL‐10, IL‐12, TNF‐α in response to bacterial invasion. These results demonstrate that this cell line provide reliable cell model system for future studies on interactions between the bovine macrophages and M ycobacterium tuberculosis .