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Sca‐1 + mesenchymal stromal cells inhibit splenic marginal zone B lymphocytes commitment through Caspase‐3
Author(s) -
Chen Yaozhen,
Yang Jialei,
Zhang HuiJie,
Fan Hong,
An Ning,
Xin Jiajia,
Li Na,
Xu Jinmei,
Yin Wen,
Wu Zhongliang,
Hu Xingbin
Publication year - 2016
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10591
Subject(s) - marginal zone , mesenchymal stem cell , stromal cell , immune system , biology , haematopoiesis , spleen , immunology , microbiology and biotechnology , b cell , antibody , cancer research , stem cell
Mesenchymal stromal cells (MSCs) have been characterized as an important component of hematopoietic niche, which are capable of modulating the immune system through interaction with a wide range of immune cells. Marginal zone B cells, one main type of mature B lymphocytes, play a central role in eliciting antibody response against pathogens. However, how MSCs and its subpopulations regulate marginal zone B cells commitment is unknown yet. In this study, we assessed the contribution of Sca‐1 + MSCs on marginal zone B cells commitment. Our results showed that Sca‐1 + MSCs inhibit the commitment of marginal zone B lymphocytes. The inhibition was exerted through lowered Caspase‐3 expression. Furthermore, we found marginal zone B lymphocytes in spleen of Caspase‐3 knockout mice decreased and Caspase‐3 knockout Sca‐1 + MSCs accounted for the MZB lymphocytes decrease. In conclusion, our investigation provided clues about Sca‐1 + MSCs regulation on the commitment of marginal zone B cells through Caspase‐3 gene.