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Contribution of α‐smooth muscle actin and extracellular matrix to the in vitro reorganization of cardiomyocyte contractile system
Author(s) -
Bildyug Natalya,
Bozhokina Ekaterina,
Khaitlina Sofia
Publication year - 2016
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10577
Subject(s) - myofibril , contractility , extracellular matrix , actin , microbiology and biotechnology , myocyte , extracellular , laminin , gene isoform , myofilament , chemistry , biology , anatomy , biochemistry , endocrinology , gene
Abstract Cardiomyocytes in culture undergo reversible rearrangement of their contractile apparatus with the conversion of typical myofibrils into the structures of non‐muscle type and the loss of contractility. Along with these transformations, the cardiomyocytes gain the capacity to synthesize extracellular matrix. Here we show that during cultivation of rat neonatal cardiomyocytes, the inherent α‐cardiac actin isoform is transiently replaced by α‐smooth‐muscle actin, whose expression is accompanied by transformation of myofibrils into stress‐fiber‐like structures. The following down‐regulation of α‐smooth muscle actin parallels restoration of myofibrillar system and correlates with the accumulation of extracellular collagen and laminin, initially missing from the cardiomyocytes culture.