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Valsartan protects HK‐2 cells from contrast media‐induced apoptosis by inhibiting endoplasmic reticulum stress
Author(s) -
Peng Pingan,
Wang Le,
Ma Qian,
Xin Yi,
Zhang Ou,
Han Hongya,
Liu Xiaoli,
Ji Qingwei,
Zhou Yujie,
Zhao Yingxin
Publication year - 2015
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10521
Subject(s) - apoptosis , unfolded protein response , endoplasmic reticulum , medicine , valsartan , pharmacology , endocrinology , cancer research , chemistry , microbiology and biotechnology , biology , biochemistry , blood pressure
Contrast‐induced acute kidney injury (CI‐AKI) is associated with increasing in‐hospital and long‐term adverse clinical outcomes in high‐risk patients undergoing percutaneous coronary intervention (PCI). Contrast media (CM)‐induced renal tubular cell apoptosis is reported to participate in this process by activating endoplasmic reticulum (ER) stress. An angiotensin II type 1 receptor (AT1R) antagonist can alleviate ER stress‐induced renal apoptosis in streptozotocin (STZ)‐induced diabetic mice and can reduce CM‐induced renal apoptosis by reducing oxidative stress and reversing the enhancement of bax mRNA and the reduction of bcl‐2 mRNA, but the effect of the AT1R blocker on ER stress in the pathogenesis of CI‐AKI is still unknown. In this study, we explored the effect of valsartan on meglumine diatrizoate‐induced human renal tubular cell apoptosis by measuring changes in ER stress‐related biomarkers. The results showed that meglumine diatrizoate caused significant cell apoptosis by up‐regulating the expression of ER stress markers, including glucose‐regulated protein 78 (GRP78), activating transcription factor 4 (ATF4), CCAAT/enhancer‐binding protein‐homologous protein (CHOP) and caspase 12, in a time‐ and dose‐dependent manner, which could be alleviated by preincubation with valsartan. In conclusion, valsartan had a potential nephroprotective effect on meglumine diatrizoate‐induced renal cell apoptosis by inhibiting ER stress.