Premium
Isolation of lung multipotent stem cells using a novel microfluidic magnetic activated cell sorting system
Author(s) -
Zeng Ling,
Qiu Lin,
Yang XueTao,
Zhou YinHan,
Du Juan,
Wang HaiYan,
Sun JianHui,
Yang Ce,
Jiang JianXin
Publication year - 2015
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10513
Subject(s) - microbiology and biotechnology , stem cell , progenitor cell , cell sorting , multipotent stem cell , biology , lung , adult stem cell , endothelial stem cell , cellular differentiation , immunology , in vitro , flow cytometry , medicine , biochemistry , gene
In recent years, more and more research has shown that the lung is an organ of regenerative potential, with several types of stem/progenitor cells undergoing proliferation and differentiation after lung injury and participating the injury repair process. Mouse lung multipotent stem cells (MLSCs) have extensive self‐renewal ability in culture and could differentiate into endothelial and lung epithelial (alveolar epithelial type 1, 2, and Clara) cells in vitro. But the research of MLSCs was limited due to its rarity. In this study, we introduced a novel microfluidic magnetic activated cell sorting system in the isolation of MLSCs. The sorted MLSCs had better viability and purity. They were identified by colony formation efficiency and differentiation ability and they have self‐renewal and differentiation capacities, highlighting their stem cell properties.