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δ‐Catenin interacts with LEF‐1 and negatively regulates its transcriptional activity
Author(s) -
He Yongfeng,
Ki Hyunkyoung,
Kim Hangun,
Kim Kwonseop
Publication year - 2015
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10465
Subject(s) - catenin , wnt signaling pathway , enhancer , beta catenin , transcriptional activity , microbiology and biotechnology , biology , cadherin , transcription factor , nucleus , chemistry , genetics , cell , gene , signal transduction
δ‐Catenin and β‐catenin belong to different subfamilies of armadillo proteins but share some common binding partners, such as E‐cadherin. This is the first study that demonstrated a novel common binding partner for δ‐catenin and β‐catenin, lymphoid enhancer factor‐1 (LEF‐1). We found that the N‐terminus of δ‐catenin (amino acids 85–325) bound to the middle region of LEF‐1 unlike β‐catenin. Overexpressed δ‐catenin entered the nucleus and inhibited LEF‐1‐mediated transcriptional activity in Bosc23 and DLD‐1 cell lines. The current study provided novel insights that will provide a better understanding of the effects of δ‐catenin on Wnt/LEF‐1‐mediated transcriptional activity.