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Changes in signal molecules and maturation promoting factor levels associate with spontaneous resumption of meiosis in rat oocytes
Author(s) -
Prasad Shilpa,
Tiwari Meenakshi,
Tripathi Anima,
Pandey Ashutosh N.,
Chaube Shail K.
Publication year - 2015
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10444
Subject(s) - maturation promoting factor , oocyte , cyclin dependent kinase 1 , cyclin b1 , guanosine , meiosis , chemistry , meiosis ii , microbiology and biotechnology , endocrinology , medicine , andrology , biology , cell cycle , biochemistry , apoptosis , embryo , gene
The present study was aimed to find out changes in signal molecules and maturation promoting factor (MPF) levels during meiotic cell cycle progression from diplotene and metaphase‐II (M‐II) arrest, a period during which oocyte achieves meiotic competency. Data suggest that high levels of adenosine 3′‐5′‐cyclic monophosphate (cAMP), guanosine 3′‐5′‐cyclic monophosphate (cGMP), and nitric oxide (NO) are associated with diplotene arrest, while reduction in their levels correlates with reduced MPF level and meiotic resumption from diplotene arrest. On the other hand, increased intracellular NO, calcium (Ca 2+ ) as well as hydrogen peroxide (H 2 O 2 ) levels correlate with decreased cAMP, Thr‐161 phosphorylated cyclin‐dependent kinase‐1 (Cdk1) as well as cyclin B1 levels. The decreased Thr‐161 phosphorylated Cdk1 and cyclin B1 level reduce MPF level leading to exit from M‐II arrest in oocytes cultured in vitro. These data suggest that the decrease of cAMP level and increase of cytosolic free Ca 2+ as well as H 2 O 2 levels associate with the reduced MPF level and meiotic resumption from diplotene arrest. On the other hand, increase of NO, cGMP, Ca 2+ as well as H 2 O 2 levels are associated with reduced MPF and spontaneous exit from M‐II arrest in rat oocytes cultured in vitro.