Premium
4‐Methylcatechol‐induced cell damage in TM4 Sertoli cells
Author(s) -
Li Chunjin,
Li Wanhong,
Chen Shuxiong,
Kang Zhichen,
Sun Lina,
Li Hongjiao,
Chen Lu,
Rao Jiahui,
Zhao Yun,
Yu Jiaxin,
Zhou Xu
Publication year - 2015
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10420
Subject(s) - sertoli cell , apoptosis , cytotoxic t cell , downregulation and upregulation , viability assay , biology , microbiology and biotechnology , cell , chemistry , spermatogenesis , gene , endocrinology , in vitro , genetics
4‐Methylcatechol (4‐MC) is one of the metabolites of quercetin, which is a potential drug for neuroprotection and tumorigenesis inhibition. This study was performed to investigate the cytotoxic effect of 4‐MC in mouse TM4 Sertoli cells. TM4 Sertoli cell viability was significantly inhibited by 4‐MC in a time‐ and dose‐dependent manner. The number of apoptotic and dead cells was significantly increased after 4‐MC treatment. Caspase 3 activity increased by prolonged exposure of TM4 Sertoli cells to 200 μM 4‐MC. The 4‐MC significantly upregulated the mRNA level of Bax gene and considerably downregulated the Bcl‐2 gene expression in a concentration‐dependent manner. Results showed that 4‐MC could induce TM4 Sertoli cell apoptosis, and the cytotoxic effect of 4‐MC on TM4 Sertoli cells may be associated with upregulated Bax gene expression, which induced caspase cascade activation.