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Chondrogenic differentiation of ChM‐I gene transfected rat bone marrow‐derived mesenchymal stem cells on 3‐dimensional poly ( L ‐lactic acid) scaffold for cartilage engineering
Author(s) -
Xing ShuangChun,
Liu Yang,
Feng Y.,
Jiang Chen,
Hu YuQiang,
Sun Wei,
Wang XinHui,
Wei ZhiYong,
Qi Min,
Liu Jing,
Zhai LiJie,
Wang ZhiQiang
Publication year - 2015
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10393
Subject(s) - chondrogenesis , mesenchymal stem cell , scaffold , transfection , chemistry , microbiology and biotechnology , bone marrow , cartilage , stem cell , tissue engineering , gene , biology , biomedical engineering , anatomy , biochemistry , immunology , medicine , genetics
We have explored the role of Chondromodulin‐I (ChM‐I) in chondrogenesis of bone marrow‐derived mesenchymal stem cells (BMSCs) in 3‐dimensional (3D) scaffold for cartilage tissue engineering. BMSCs of Sprague Dawley (SD) rats were cultured on poly‐( L ‐lactic acid) [PLLA] scaffolds with different pore sizes (80–200 μm, 200–450 μm) with or without surface modification by chitosan. Cell viability, proliferation, and morphology were measured using confocal microscope and the CCK‐8 method. Untransfected BMSCs, BMSCs expressing pcDNA3.1(+), BMSCs expressing plasmid pcDNA3.1 (+)/ChM‐I were cultured on 3D scaffolds in standard growth medium or transforming growth factor‐β1 (TGF‐β1) supplemented chondrogenic induction medium in vitro for 3 weeks and the expression of collagen type II was determined. Cell‐scaffolds constructs were implanted subcutaneously for 3 months in vivo . BMSCs had a higher viability and proliferation in PLLA scaffolds of pore size 200–450 μm than that of 80–200 μm, and surface modification with chitosan did not enhance cell attachment. The ChM‐I gene enhanced chondrogenesis and increased collagen type II synthesis. Immunohistochemistry from in vivo study showed enhanced cartilage regeneration in BMSCs expressing pcDNA3.1 (+)/ChM‐I on 3D PLLA scaffolds. It also demonstrated that TGF‐β1 might promote chondrogenesis of rat BMSCs by synergizing with the ChM‐I gene. ChM‐I could be beneficial to future applications in cartilage repair.