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Pulmonary surfactant expression analysis—Role of cell–cell interactions and 3‐D tissue‐like architecture
Author(s) -
Nandkumar Maya A.,
Ashna U.,
Thomas Lynda V.,
Nair Prabha D.
Publication year - 2015
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10389
Subject(s) - pulmonary surfactant , scaffold , in vivo , cell culture , chemistry , microbiology and biotechnology , cell , in vitro , cell adhesion , biophysics , adhesion , 3d cell culture , biomedical engineering , biology , biochemistry , organic chemistry , medicine , genetics
Surfactant production is important in maintaining alveolar function both in vivo and in vitro, but surfactant expression is the primary property lost by alveolar Type II Pneumocytes in culture and its maintenance is a functional requirement. To develop a functional tissue‐like model, the in vivo cell–cell interactions and three dimensional architecture has to be reproduced. To this end, 3D button‐shaped synthetic gelatin vinyl acetate (GeVAc) co‐polymer scaffold was seeded with different types of lung cells. Functionality of the construct was studied under both static and dynamic conditions. The construct was characterized by Environmental Scanning Electron and fluorescent microscopy, and functionality of the system was analyzed by studying mRNA modulations of all four surfactant genes A, B, C, and D by real time‐PCR and varying culture conditions. The scaffold supports alveolar cell adhesion and maintenance of cuboidal morphology, and the alveolar‐specific property of surfactant synthesis, which would otherwise be rapidly lost in culture. This is a novel 3D system that expresses all 4 surfactants for a culture duration of 3 weeks.