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Retinoic acid can induce mouse embryonic stem cell R1/E to differentiate toward female germ cells while oleanolic acid can induce R1/E to differentiate toward both types of germ cells
Author(s) -
Wan Qian,
Lu Hua,
Wu LinTao,
Liu Xia,
Xiang JunBei
Publication year - 2014
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10380
Subject(s) - retinoic acid , germ , embryonic stem cell , microbiology and biotechnology , germ cell , p19 cell , stem cell , biology , germ line development , chemistry , adult stem cell , cell culture , genetics , gene
Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC‐R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC‐R1/E and EBs were allowed to attach to the bottoms of normal cell‐culturing plate and grow. Then, different compounds including RA, OA and so on were respectively added to induce MESC‐R1/E to differentiate. After 72 h, microscopy images were taken for all interventions, then total RNAs were extracted, cDNAs were synthesized and real‐time fluorescence quantitative PCR (qPCR) was performed to detect the transcriptional expression patterns of 11 reproductive‐differentiation‐related genes for different compounds respectively. During the data analysis, it was found RA significantly up‐regulated the expression levels of GDF‐9 , Stra8 , SCP3 , Mvh , ZP1 , ZP2, and ZP3 , while significantly down‐regulated the levels of Itag6 and Itgb1 , and the level of Oct‐4 was down‐regulated insignificantly, while the level of TP2 was up‐regulated insignificantly; OA significantly up‐regulated the expression levels of Stra8 , SCP3 , Mvh , ZP1 , ZP2 , Itgb1, and TP2 , and the levels of Oct‐4 , GDF‐9 , ZP3, and Itga6 were up‐regulated insignificantly. The data showed that RA can induce MESC‐R1/E to differentiate toward female germ cells while OA can induce MESC‐R1/E to differentiate toward male and female germ cells.