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Knockdown of CUG‐binding protein 1 induces apoptosis of human laryngeal cancer cells
Author(s) -
Zhou Yi,
Ma Hongzhi,
Fang Jugao,
Lian Meng,
Feng Ling,
Wang Ru
Publication year - 2014
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10356
Subject(s) - gene knockdown , apoptosis , small hairpin rna , biology , microbiology and biotechnology , cancer cell , cancer research , cell cycle , cell growth , cancer , genetics
To investigate the role of CUG‐binding protein 1 (CUGBP1) in human laryngeal cancer, we employed lentivirus‐mediated short hairpin RNA (shRNA) to knockdown CUGBP1 expression in Hep‐2 cells. Depletion of CUGBP1 remarkably inhibited the proliferation of Hep‐2 cells. CUGBP1 knockdown induced cell cycle arrest in S phase, especially in the sub‐G1 phase, representing apoptotic cells. Knockdown of CUGBP1 in Hep‐2 cells markedly increased the expression of LIP and cleavage of PARP, which could contribute to apoptosis. Thus CUGBP1 has a critical role in modulating cell growth and apoptosis, and serves as a potential therapeutic target in laryngeal cancer.