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RIG‐I knockdown impedes neurogenesis in a murine model of Japanese encephalitis
Author(s) -
Mukherjee Sriparna,
Ghosh Sourish,
Nazmi Arshed,
Basu Anirban
Publication year - 2015
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10354
Subject(s) - gene knockdown , neurogenesis , progenitor cell , biology , neural stem cell , virus , in vivo , japanese encephalitis , retinoic acid , virology , microbiology and biotechnology , cell growth , encephalitis , cell culture , stem cell , genetics
Retinoic acid inducible gene I (RIG‐I) is a well established pattern recognition receptor (PRR) in neurons infected with Japanese encephalitis virus (JEV) as reported previously from our laboratory. Japanese encephalitis (JE) virus infection in brain has been shown to decrease the proliferation of neural stem/progenitor cells (NSPCs) which has its implications in neurological sequelae in JE survivors. We have found that ablation of RIG‐I both in vivo and in vitro models results in significant decrease in NSPC proliferation post JEV infection. We hypothesize that knockdown of RIG‐I diminishes the expression of antiviral molecules resulting in an increase in viral replication, which in turn results in enhancement of the expression of cell cycle inhibitors, hence affecting the proliferation of NSPCs.