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Growth factors have a protective effect on neomycin‐induced hair cell loss
Author(s) -
Lou Xiangxin,
Yuan Huihua,
Xie Jing,
Wang Xianliu,
Yang Liangliang,
Zhang Yanzhong
Publication year - 2015
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10347
Subject(s) - organ of corti , neomycin , hair cell , ototoxicity , cochlea , growth factor , epidermal growth factor , progenitor cell , basic fibroblast growth factor , biology , microbiology and biotechnology , fibroblast growth factor , inner ear , regeneration (biology) , endocrinology , medicine , andrology , immunology , anatomy , stem cell , cell culture , biochemistry , genetics , chemotherapy , receptor , cisplatin , antibiotics
We have demonstrated that selected growth factors are involved in regulating survival and proliferation of progenitor cells derived from the neonatal rat organ of Corti (OC). The protective and regenerative effects of these defined growth factors on the injured organ of Corti were therefore investigated. The organ of Corti dissected from the Wistar rat pups (P3‐P5) was split into apical, middle, and basal parts, explanted and cultured with or without neomycin and growth factors. Insulin‐like growth factor‐1 (IGF‐1), fibroblast growth factor‐2 (FGF‐2), and epidermal growth factor (EGF) protected the inner hair cells (IHCs) and outer hair cells (OHCs) from neomycin ototoxicity. Using EGF, IGF‐1, and FGF‐2 alone induced no protective effect on the survival of auditory hair cells. Combining 2 growth factors (EGF + IGF‐1, EGF + FGF‐2, or IGF‐1 + FGF‐2) gave statistically protective effects. Similarly, combining all three growth factors effectively protected auditory hair cells from the ototoxic insult. None of the growth factors induced regeneration of hair cells in the explants injured with neomycin. Thus various combinations of the three defined factors (IGF‐1, FGF‐2, and EGF) can protect the auditory hair cells from the neomycin‐induced ototoxic damage, but no regeneration was seen. This offers a possible novel approach to the treatment of hearing loss.