Premium
Effects of carbon‐ion beam irradiation on the angiogenic response in lung adenocarcinoma A549 cells
Author(s) -
Liu Yuanyuan,
Liu Yang,
Zhang Hong,
Sun Chao,
Zhao Qiuyue,
Di Cixia,
Li Hongyan,
Gan Lu,
Wang Yali
Publication year - 2014
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1002/cbin.10327
Subject(s) - angiogenesis , a549 cell , vascular endothelial growth factor , basic fibroblast growth factor , endothelial stem cell , cancer research , fibroblast growth factor , carbon ion radiotherapy , chemistry , fibroblast , biology , growth factor , microbiology and biotechnology , cell , medicine , in vitro , radiation therapy , biochemistry , vegf receptors , receptor
Radiotherapy has been focused mainly on killing cancer cells, and little attention has been paid to the process supporting tumor growth and metastasis, including the process of angiogenesis. To investigate the effects of carbon‐ion irradiation on angiogenesis in lung cancer cells, we examined the expression of vascular endothelial growth factor and basic fibroblast growth factor in the tumor conditioned medium (TCM) of A549 cells exposed to carbon‐ion or X‐ray irradiation, as well as endothelial cell growth, invasion, and tube formation induced by TCM. No changes in vascular endothelial growth factor secretion were detected in the TCM of A549 cells exposed to carbon‐ion irradiation at 2 or 4 Gy, whereas 1 Gy of irradiation significantly decreased vascular endothelial growth factor and basic fibroblast growth factor levels. Carbon‐ion irradiation at 1 Gy inhibited endothelial cell invasion and tube formation. The TCM from A549 cells irradiated with X‐ray promoted angiogenesis, whereas the TCM of A549 cells exposed to carbon‐ion irradiation at 2 or 4 Gy had no effect. These findings suggest that carbon‐ion irradiation at 1 Gy significantly suppressed the process of angiogenesis in vitro by inhibiting endothelial cell invasion and tube formation, which are related to vascular endothelial growth factor and basic fibroblast growth factor production.